本书用英语介绍了利用基因进行治疗的方法、原理。主要是对多咱疾病的治疗又有新的突破，采用基因学是一个很主要的方面，又可预防多种传染病和遗传病。适合专业人员阅读。

Preface

Contributors

1.Methods for Gene Transfer Using DNA-Adenovirus Conjugates,DavidT.Curiel

2.Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors,Jeffrey S.Bartlett and Richard J.Samulski

3.Generation of High-Titer,Helper-Free Retroviruses by Transient Transfection,Warren S.Pear,Martin L Scott,and Garry P.Nolan

4.Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers,Isabelle Riviere and Michel Sadelain

5.Development of Replication-Defective Herpes Simplex Virus Vectors,David Krisky,Peggy Marconi,William F.Goins,and Joseph C.Glorioso

6.Methods for the Use of Poliovirus Vectors for Gene Delivery,Cassy D.Morrow,David C.Ansardi,and Donna C.Porter

7.Methods for the Construction of Human Papillomavirus Vectors,Saleem A.Khan and Francis M.Sverdrup

8.Methods for Liposome-Mediated Gene Transfer to the Arterial Wall,Elizabeth G.Nanel,Zhi-yong Yang,Hong San,Dianne P.Carr,and Gary J.Nabel

9.Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes,Mark A.Findeis,Catherine H.Wu,and George Y.Wu

10.Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium,John C.Olsen,Larry G.Johnson,and James R.Yankaskas

……

Index

Safety procedures shousld be observed in infecting animals with the viral solutions in the animal room.Use 10% bleach or Bacdown solution to clean the laminar flow hood before and after the operation.